Rapid degradation of the presequence of the F1β precursor of the ATP synthase inside mitochondria

被引：16

作者：

Ståhl, A ^{[1
]}

Pavlov, PF ^{[1
]}

Szigyarto, C ^{[1
]}

Glaser, E ^{[1
]}

机构：

[1] Stockholm Univ, Dept Biochem, Arrhenius Labs Nat Sci, S-10691 Stockholm, Sweden

来源：

BIOCHEMICAL JOURNAL | 2000年 / 349卷

关键词：

protein import; proteolysis; signal peptide;

D O I：

10.1042/bj3490703

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the fate of the presequence of an overexpressed protein derived from the precursor of the F(1)beta subunit of ATP synthase after import and processing in mitochondria. Our studies revealed a rapid degradation of the presequence inside mitochondria catalysed by matrix-located protease(s). In contrast, the mature portion of the precursor was not degraded. This is the first experimental evidence of the rapid degradation of the F(1)beta a mitochondrial presequence in organello after in vitro import and processing.

引用

页码：703 / 707

页数：5

共 29 条

[1] Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20 [J].

Abe, Y ;

Shodai, T ;

Muto, T ;

Mihara, K ;

Torii, H ;

Nishikawa, S ;

Endo, T ;

Kohda, D .

CELL, 2000, 100 (05) :551-560

[2]

ARRETZ M, 1994, J BIOL CHEM, V269, P4959

[3] Human ClpP protease: CDNA sequence, tissue-specific expression and chromosomal assignment of the gene [J].

Bross, P ;

Andresen, BS ;

Knudsen, I ;

Kruse, TA ;

Gregersen, N .

FEBS LETTERS, 1995, 377 (02) :249-252

[4] PROTEINASE YSCD (OLIGOPEPTIDASE YSCD) - STRUCTURE, FUNCTION AND RELATIONSHIP OF THE YEAST ENZYME WITH MAMMALIAN THIMET OLIGOPEPTIDASE (METALLOENDOPEPTIDASE, EP-24.15) [J].

BUCHLER, M ;

TISLJAR, U ;

WOLF, DH .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 219 (1-2) :627-639

[5] LATENT MEMBRANE PERTURBATION ACTIVITY OF A MITOCHONDRIAL PRECURSOR PROTEIN IS EXPOSED BY UNFOLDING [J].

ENDO, T ;

SCHATZ, G .

EMBO JOURNAL, 1988, 7 (04) :1153-1158

[6] CYTOSOLIC AND MITOCHONDRIAL SURFACE FACTOR-INDEPENDENT IMPORT OF A SYNTHETIC PEPTIDE INTO MITOCHONDRIA [J].

FURUYA, S ;

MIHARA, K ;

AIMOTO, S ;

OMURA, T .

EMBO JOURNAL, 1991, 10 (07) :1759-1766

[7] Mitochondrial protein import in plants -: Signals, Sorting targeting, processing and regulation [J].

Glaser, E ;

Sjöling, S ;

Tanudji, M ;

Whelan, J .

PLANT MOLECULAR BIOLOGY, 1998, 38 (1-2) :311-338

[8]

GLASER SM, 1990, J BIOL CHEM, V265, P8817

[9]

GLASER SM, 1990, J BIOL CHEM, V265, P8808

[10] Impact of the presequence of a mitochondrium-targeted precursor, preadrenodoxin, on folding, catalytic activity, and stability of the protein in vitro [J].

Goder, V ;

Beckert, V ;

Pfeil, W ;

Bernhardt, R .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1998, 359 (01) :31-41

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