Homogenous time resolved fluorescence assay to identify modulators of cap-dependent translation initiation

被引：5

作者：

Cencic, Regina

Yan, Yifei

Pelletier, Jerry

机构：

[1] McGill Univ, Dept Biochem, Montreal, PQ H3G 1Y6, Canada

[2] McGill Univ, Ctr Canc, Montreal, PQ H3G 1Y6, Canada

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2007年 / 10卷 / 03期

关键词：

D O I：

10.2174/138620707780126688

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Eukaryotic initiation factor (eIF) 4F plays a key role in recruiting 40S ribosomes and associated factors to mRNA templates during translation initiation. The function of this heterotrimeric complex is to deliver an RNA helicase to the 5' cap proximal region of mRNAs in preparation for ribosome binding. To study the interaction between subunits of this complex, as well as identify small molecules that could interfere with their association, we developed a time resolved fluorescence assay that allows monitoring of interactions between two subunits of eIF4F. We have performed a small molecule chemical screen of > 73,000 compounds using this assay.

引用

页码：181 / 188

页数：8

共 36 条

[1] Depletion of intracellular Ca2+ stores, phosphorylation of eIF2α, and sustained inhibition of translation initiation mediate the anticancer effects of clotrimazole [J].