Directed mass spectrometry: towards hypothesis-driven proteomics

被引:74
作者
Schmidt, Alexander [1 ]
Claassen, Manfred [1 ]
Aebersold, Ruedi [1 ,2 ,3 ,4 ]
机构
[1] ETH, Inst Mol Syst Biol, CH-8093 Zurich, Switzerland
[2] ETH, Competence Ctr Syst Physiol & Metab Dis, CH-8093 Zurich, Switzerland
[3] Inst Syst Biol, Seattle, WA 98103 USA
[4] Univ Zurich, Fac Sci, CH-8006 Zurich, Switzerland
基金
瑞士国家科学基金会;
关键词
POSTTRANSLATIONAL MODIFICATIONS; ABSOLUTE QUANTIFICATION; QUANTITATIVE PROTEOMICS; PROTEIN IDENTIFICATION; COMPLEX-MIXTURES; MS; PEPTIDES; PREDICTION; DISCOVERY; STRATEGY;
D O I
10.1016/j.cbpa.2009.08.016
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To date, the vast majority of the proteomic data sets collected by mass spectrometry (MS) have been generated by nondirected methods, whereby the identified precursor ions are stochastically selected for sequencing from complex sample mixtures. Recently, new MS approaches have been developed in which the mass spectrometer is directed to select and fragment sets of precursor ions that represent the most informative peptides in a sample mixture. These directed MS methods have shown superior performance for the fast, sensitive, and highly reproducible generation of consistent data sets at low redundancy. In this manuscript we summarize recent technical advances in directed MS and discuss important applications to quantitative proteomics.
引用
收藏
页码:510 / 517
页数:8
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