Detection of noroviruses in raspberries associated with a gastroenteritis outbreak

被引:141
作者
Le Guyader, FS
Mittelholzer, C
Haugarreau, L
Hedlund, KO
Alsterlund, R
Pommepuy, M
Svensson, L
机构
[1] IFREMER, Lab Microbiol, F-44311 Nantes 03, France
[2] Swedish Inst Infect Dis Control, Dept Virol, S-17182 Solna, Sweden
[3] Inst Marine Res Austevoll, N-5392 Storebo, Norway
[4] Reg Ctr Communicable Dis Control, S-29185 Kristianstad, Sweden
[5] Linkoping Univ, Dept Mol Virol, S-58185 Linkoping, Sweden
关键词
norovirus; gastroenteritis; food analysis; reversetranscriptase polymerase chain reaction; sequence analysis;
D O I
10.1016/j.ijfoodmicro.2004.04.018
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Following an acute foodborne gastroenteritis outbreak in southern Sweden, stool specimens from five of nine ill patients were found positive for norovirus using reverse-transcriptase polymerase chain reaction. Epidemiological data pointed to raspberry cakes as the source of the outbreak. Using a combination of generic and patient-specific primers and novel food analysis methodology (with extraction efficiency control and inhibitor removal), norovirus strains from two different genogroups were directly identified in the contaminated raspberries. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:179 / 186
页数:8
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