Analysis of the proteins targeted by CDSP32, a plastidic thioredoxin participating in oxidative stress responses
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作者:
Rey, P
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CEN Cadarache, DSV, DEVM, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, FranceCEN Cadarache, DSV, DEVM, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
Rey, P
[1
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Cuiné, S
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机构:CEN Cadarache, DSV, DEVM, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
Cuiné, S
Eymery, F
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机构:CEN Cadarache, DSV, DEVM, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
Eymery, F
Garin, J
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机构:CEN Cadarache, DSV, DEVM, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
Garin, J
Court, M
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机构:CEN Cadarache, DSV, DEVM, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
Court, M
Jacquot, JP
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机构:CEN Cadarache, DSV, DEVM, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
Jacquot, JP
Rouhier, N
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机构:CEN Cadarache, DSV, DEVM, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
Rouhier, N
Broin, M
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机构:CEN Cadarache, DSV, DEVM, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
Broin, M
机构:
[1] CEN Cadarache, DSV, DEVM, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
[2] CEA Grenoble, Lab Chim Prot, ERM CEA, INSERM 0201, F-38054 Grenoble 9, France
[3] Univ Nancy 1, Inst Natl Rech Agron, UMR 1136, F-54506 Vandoeuvre Les Nancy, France
The chloroplastic drought-induced stress protein of 32 kDa (CDSP32) is a thioredoxin induced by environmental stress conditions. To gain insight into the function of CDSP32, we applied two strategies to analyze its targets. First, using affinity chromatography with an immobilized CDSP32 active site mutant, we identified six plastidic targets of CDSP32. Three of them are involved in photosynthetic processes: ATP-ase gamma-subunit, Rubisco and aldolase. The three others participate in the protection against oxidative damage: two peroxiredoxins, PrxQ and the BAS1 2-Cys peroxiredoxin, and a B-type methionine sulfoxide reductase. Then, we developed a novel strategy to trap targets directly in leaf extracts. The method, based on co-immunoprecipitation using extracts from plants overexpressing Wt CDSP32 or CDSP32 active site mutant, confirmed the interaction in vivo between CDSP32 and the PrxQ and BAS1 peroxiredoxins. We showed that CDSP32 is able to form heterodimeric complexes with PrxQ and that the peroxiredoxin displays CDSP32-dependent peroxidase activity. Under photooxidative stress induced by methyl viologen, plants overexpressing CDSP32 active site mutant exhibit decreased maximal PSII photochemical efficiency and retain much less chlorophyll compared with Wt plants and with plants overexpressing Wt CDSP32. We propose that the increased sensitivity results from trapping in planta of the targets involved in the protection against oxidative damage. We conclude that CDSP32, compared with other plant thioredoxins, is a thioredoxin more specifically involved in plastidic responses against oxidative stress.
机构:
Karolinska Inst, Med Nobel Inst Biochem, Dept Med Biochem & Biophys, SE-17177 Stockholm, SwedenKarolinska Inst, Med Nobel Inst Biochem, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden
Arnér, ESJ
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机构:
Holmgren, A
[J].
EUROPEAN JOURNAL OF BIOCHEMISTRY,
2000,
267
(20):
: 6102
-
6109
机构:
CEA Cadarache, Direct Sci Vivant, Dept Ecophysiol Vegetale & Microbiol, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, FranceCEA Cadarache, Direct Sci Vivant, Dept Ecophysiol Vegetale & Microbiol, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
Broin, M
Rey, P
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机构:
CEA Cadarache, Direct Sci Vivant, Dept Ecophysiol Vegetale & Microbiol, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, FranceCEA Cadarache, Direct Sci Vivant, Dept Ecophysiol Vegetale & Microbiol, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
机构:
CEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, FranceCEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, France
Broin, M
Besse, I
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机构:
CEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, FranceCEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, France
Besse, I
Rey, P
论文数: 0引用数: 0
h-index: 0
机构:
CEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, FranceCEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, France
机构:
Karolinska Inst, Med Nobel Inst Biochem, Dept Med Biochem & Biophys, SE-17177 Stockholm, SwedenKarolinska Inst, Med Nobel Inst Biochem, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden
Arnér, ESJ
论文数: 引用数:
h-index:
机构:
Holmgren, A
[J].
EUROPEAN JOURNAL OF BIOCHEMISTRY,
2000,
267
(20):
: 6102
-
6109
机构:
CEA Cadarache, Direct Sci Vivant, Dept Ecophysiol Vegetale & Microbiol, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, FranceCEA Cadarache, Direct Sci Vivant, Dept Ecophysiol Vegetale & Microbiol, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
Broin, M
Rey, P
论文数: 0引用数: 0
h-index: 0
机构:
CEA Cadarache, Direct Sci Vivant, Dept Ecophysiol Vegetale & Microbiol, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, FranceCEA Cadarache, Direct Sci Vivant, Dept Ecophysiol Vegetale & Microbiol, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France
机构:
CEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, FranceCEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, France
Broin, M
Besse, I
论文数: 0引用数: 0
h-index: 0
机构:
CEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, FranceCEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, France
Besse, I
Rey, P
论文数: 0引用数: 0
h-index: 0
机构:
CEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, FranceCEA, Dept Ecophysiol Vegetale & Microbiol, DSV, Lab Ecophysiol Photosynthese, F-13108 St Paul Les Durance, France