Stereospecific assignment of H5′ and H5" in a (5′R)-/(5′S)-deuterium-labeled DNA decamer for 3JHH determination and unambiguous NOE assignments

被引：16

作者：

Kojima, C

Kawashima, E

Toyama, K

Ohshima, K

Ishido, Y

Kainosho, M

Kyogoku, Y ^{[1
]}

机构：

[1] Osaka Univ, Inst Prot Res, Suita, Osaka 565, Japan

[2] Tokyo Metropolitan Univ, Fac Sci, Dept Chem, Tokyo 19203, Japan

[3] Tokyo Univ Pharm & Life Sci, Fac Pharm, Lab Pharmaceut Chem, Tokyo 19203, Japan

来源：

JOURNAL OF BIOMOLECULAR NMR | 1998年 / 11卷 / 01期

基金：

日本学术振兴会;

关键词：

backbone angle gamma; DNA; stereospecific assignment of 5 '-methylene protons; stereospecific deuterium labeling;

D O I：

10.1023/A:1008286925516

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The stereoselective deuterium labeling at the 5' methylene protons of the ribose ring recently developed by Kawashima et al. [1995, Tetrahedron Lett., 36, 6699-6700] enabled the assignment of pro-R and pro-S protons at the 5' position. The deuterium-labeled nucleotides, [(5'S)-H-2]- and [(5'R)-H-2]-diastereomers, in an approximate ratio of 2:1, were incorporated in the decamer 5'-d(GCATTAATGC)-3', Thus, both pro-R and pro-S methylene proton signals without geminal coupling appeared in the NOESY and DQF-COSY spectra. Complete stereospecific assignments and simplified spin systems enabled the determination of 15 (3)J coupling constants between H4' and H5'/H5 ", and the unambiguous assignment of 135 NOESY cross peaks originating from H4'/H5'/H5 " resonances.

引用

页码：103 / 109

页数：7

共 28 条

[1] THE 3-DIMENSIONAL STRUCTURE OF A DNA HAIRPIN IN SOLUTION - 2-DIMENSIONAL NMR-STUDIES AND STRUCTURAL-ANALYSIS OF D(ATCCTATTTATAGGAT) [J].