Characterization of zetin 1/rBSPRY, a novel binding partner of 14-3-3 proteins

被引：7

作者：

Birkenfeld, J ^{[1
]}

Kartmann, B ^{[1
]}

Anliker, B ^{[1
]}

Ono, K ^{[1
]}

Schlötcke, B ^{[1
]}

Betz, H ^{[1
]}

Roth, D ^{[1
]}

机构：

[1] Max Planck Inst Brain Res, Dept Neurochem, D-60528 Frankfurt, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 302卷 / 03期

关键词：

14-3-3; proteins; actin; B-box; SPRY domain; brain expression;

D O I：

10.1016/S0006-291X(03)00182-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

14-3-3 proteins are ubiquitously expressed proteins which serve as central adaptors in different signal transduction cascades. In this study, yeast two-hybrid screening of a rat brain cDNA library identified a novel gene product termed zetin 1/rBSPRY that interacts with 14-3-3zeta. The zetin 1/rBSPRY gene is ubiquitously expressed in a variety of rat tissues, with highest expression being found in testis. In adult brain, high levels of zetin 1/rBSPRY mRNA were observed in the hippocampus, cereberal cortex, and piriform cortex. Biochemical studies confirmed zetin 1/rBSPRY to interact with 14-3-3zeta. Transient co-transfection in COS 7 cells caused a partial redistribution of zetin 1/rBSPRY into 14-3-3zeta enriched submembranous foci at leading edges. Our results suggest a role for zetin 1/rBSPRY-14-3-3 interactions at specialized submembrane domains. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：526 / 533

页数：8

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