Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction

被引：30

作者：

Koyama, T

Okada, T

Kitajima, S

Ohme-Takagi, M

Shinshi, H

Sato, F ^{[1
]}

机构：

[1] Kyoto Univ, Dept Plant Gene & Totipotency, Grad Sch Biostudies, Div Integrated Life Sci, Kyoto 6068502, Japan

[2] Kyoto Univ, Div Appl Life Sci, Grad Sch Agr, Kyoto 6068502, Japan

[3] Natl Inst Adv Ind Sci & Technol, Gene Funct Res Lab, Tsukuba, Ibaraki 3050046, Japan

[4] Natl Inst Adv Ind Sci & Technol, Inst Mol & Cell Biol, Tsukuba, Ibaraki 3050046, Japan

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2003年 / 54卷 / 385期

基金：

日本学术振兴会;

关键词：

degradation; ethylene responsive factor (ERF); repressor; transient assay; two-hybrid system; ubiquitin-conjugating enzyme (UBC);

D O I：

10.1093/jxb/erg136

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Tobacco ETHYLENE-RESPONSIVE FACTOR3 (ERF3) is a member of the ERF-domain transcription factors and has a transcriptional repressor activity, whereas other ERF proteins show activation activity. To understand the regulation of ERF3-repressor activity, protein(s) were screened which interact with ERF3 in a yeast two-hybrid system. A partial sequence (1218) of NtUBC2, a tobacco ubiquitin-conjugating enzyme was isolated. This B8 specifically interacted with ERF3 in the yeast two-hybrid system. Further analyses revealed that the region unique to ERF3 interacted with B8. The physiological functions of NtUBC2 and the stability of ERF3 are discussed in relation to the regulation of the repression activity of ERF3.

引用

页码：1175 / 1181

页数：7

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