Developmental pathway for biofilm formation in curli-producing Escherichia coli strains:: role of flagella, curli and colanic acid

被引:348
作者
Prigent-Combaret, C [1 ]
Prensier, G
Le Thi, TT
Vidal, O
Lejeune, P
Dorel, C
机构
[1] Inst Natl Sci Appl, Lab Microbiol & Genet, CNRS, UMR 5577, F-69621 Villeurbanne, France
[2] Univ Clermont Ferrand, Lab Biol Comparee Protistes, UPES A CNRS 6023, F-63177 Aubiere, France
关键词
D O I
10.1046/j.1462-2920.2000.00128.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This work was performed to establish a model describing bacterial surface structures involved in biofilm development, in curli-overproducing Escherichia coli K-12 strains, at 30 degrees C, and in minimal growth medium. Using a genetic approach, in association with observations of sessile communities by light and electron microscopic techniques, the role of protein surface structures, such as flagella and curli, and saccharidic surface components, such as the E. coli exopolysaccharide, colanic acid, was determined. We show that, in the context of adherent ompR234 strains, (i) flagellar motility is not required for initial adhesion and biofilm development; (ii) both primary adhesion to inert surfaces and development of multilayered cell clusters require curli synthesis; (iii) curli display direct interactions with the substratum and form interbacterial bundles, allowing a cohesive and stable association of cells; and (iv) colanic acid does not appear critical for bacterial adhesion and further biofilm development but contributes to the biofilm architecture and allows for the formation of voluminous biofilms.
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页码:450 / 464
页数:15
相关论文
共 65 条
[1]   CHEMOTAXIS IN BACTERIA [J].
ADLER, J .
SCIENCE, 1966, 153 (3737) :708-&
[2]   THE ROLE OF CAPSULAR POLYSACCHARIDE K21B OF KLEBSIELLA AND OF THE STRUCTURALLY RELATED COLANIC-ACID POLYSACCHARIDE OF ESCHERICHIA-COLI IN RESISTANCE TO PHAGOCYTOSIS AND SERUM KILLING [J].
ALLEN, PM ;
FISHER, D ;
SAUNDERS, JR ;
HART, CA .
JOURNAL OF MEDICAL MICROBIOLOGY, 1987, 24 (04) :363-370
[3]  
ALLISON DG, 1987, J GEN MICROBIOL, V133, P1319
[4]   A STAINING TECHNIQUE FOR ATTACHED BACTERIA AND ITS CORRELATION TO EXTRACELLULAR CARBOHYDRATE PRODUCTION [J].
ALLISON, DG ;
SUTHERLAND, IW .
JOURNAL OF MICROBIOLOGICAL METHODS, 1984, 2 (02) :93-99
[5]   ATG VECTORS FOR REGULATED HIGH-LEVEL EXPRESSION OF CLONED GENES IN ESCHERICHIA-COLI [J].
AMANN, E ;
BROSIUS, J .
GENE, 1985, 40 (2-3) :183-190
[6]  
[Anonymous], 1993, STARVATION BACTERIA
[7]  
ARNQVIST A, 1992, MOL MICROBIOL, V6, P2443
[8]   SIGMA(S)-DEPENDENT GROWTH-PHASE INDUCTION OF THE CSGBA PROMOTER IN ESCHERICHIA-COLI CAN BE ACHIEVED IN-VIVO BY SIGMA(70) IN THE ABSENCE OF THE NUCLEOID-ASSOCIATED PROTEIN H-NS [J].
ARNQVIST, A ;
OLSEN, A ;
NORMARK, S .
MOLECULAR MICROBIOLOGY, 1994, 13 (06) :1021-1032
[9]   Thin aggregative fimbriae enhance Salmonella enteritidis biofilm formation [J].
Austin, JW ;
Sanders, G ;
Kay, WW ;
Collinson, SK .
FEMS MICROBIOLOGY LETTERS, 1998, 162 (02) :295-301
[10]  
BARDONNET N, 1992, FEMS MICROBIOL LETT, V93, P243, DOI 10.1016/0378-1097(92)90469-5