Occurrence and taxonomic significance of oxo-fatty acids in lipopolysaccharides from members of Mesorhizobium

被引:7
作者
Choma, A
Urbanik-Sypniewska, T
Russa, R
Kutkowska, J
Mayer, H
机构
[1] Marie Curie Sklodowska Univ, Dept Gen Microbiol, PL-20033 Lublin, Poland
[2] Max Planck Inst Immunobiol, D-7800 Freiburg, Germany
关键词
Mesorhizobium; lipopolysaccharides; fatty acids; 27-oxooctacosanoic acid; 4-oxo fatty acids;
D O I
10.1016/S0723-2020(00)80003-X
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Lipopolysaccharides (LPSs) isolated from seven strains of Mesorhizobium were studied for the presence of fatty acids with particular attention for 27-oxooctacosanoic acid and 4-oxo fatty acids. The LPSs from all analysed strains contained various amounts of 27-oxo-28:0 and all of them, with the exception of Mesorhizobium tianshanense, contained also 4-oxo fatty acids (4-oxo-20:0, 4-oxo-i-21:0, 4-oxo22:0). The group of amide-linked fatty acids consisted of a wide range of 3-hydroxylated and 4-oxo fatty acids whereas all the nonpolar as well as the (omega-1) hydroxylated long-chain acids and the 27-oxo-28:0 fatty acids were ester-linked. The characteristic spectrum of 3-hydroxy fatty acids and presence of 27-OH-28:0 as well as 27-oxo-28:0 acid in LPSs of Mesorhizobium showed that these strains were closely related. Therefore the lipid A fatty acid pattern could be a useful chemotaxonomic marker which helps to isolate the Mesorhizobium group from rhizobium bacteria during the classification process.
引用
收藏
页码:185 / 190
页数:6
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