RAPD markers linked to a clubroot-resistance locus in Brassica rapa L.

被引：70

作者：

Kuginuki, Y ^{[1
]}

Ajisaka, H ^{[1
]}

Yui, M ^{[1
]}

Yoshikawa, H ^{[1
]}

Hida, K ^{[1
]}

Hirai, M ^{[1
]}

机构：

[1] Natl Res Inst Vegetables Ornamental Plants & Tea, Ano, Mie 51423, Japan

来源：

EUPHYTICA | 1997年 / 98卷 / 03期

关键词：

Chinese cabbage; doubled haploid; microspore culture; Plasmodiophora brassicae; Random Amplified Polymorphic DNA;

D O I：

10.1023/A:1003147815692

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Linkage of random amplified polymorphic DNA (RAPD) markers with resistance genes to clubroot (Plasmodiophora brassicae Wor.) in Brassica rapa L. was studied in a doubled haploid (DH population obtained by microspore culture. Thirty-six DH lines were obtained from F-1 plants from a cross between susceptible 'Homei P09' and resistant 'Siloga S2' plants. 'Homei P09' was a DH line obtained by microspore culture of the Chinese cabbage variety 'Homei', which is highly responsive in microspore culture. The resistant line 'Siloga S2' was obtained by two rounds of selfing of the fodder turnip 'Siloga'. Three RAPD markers, RA12-75A, WE22B and WE49B, were found to be linked to a clubroot-resistance locus. These three markers were linked in the DH lines and an F-2 population and should be useful for marker-assisted selection in breeding programs.

引用

页码：149 / 154

页数：6

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