The role of xylulokinase in Saccharomyces cerevisiae xylulose catabolism

被引：73

作者：

Richard, P ^{[1
]}

Toivari, MH ^{[1
]}

Penttilä, M ^{[1
]}

机构：

[1] VTT Biotechnol, FIN-02044 Espoo, Finland

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 190卷 / 01期

关键词：

xylulokinase; D-xylulose; D-ribulose; xylose fermentation; Saccharomyces cerevisiae;

D O I：

10.1016/S0378-1097(00)00312-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Many yeast species have growth rates on D-xylulose of 25-130% of those on glucose, but for Saccharomyces cerevisiae this ratio is only about 6%. The xylulokinase reaction has been proposed to be the rate-limiting step in the D-xylulose fermentation with S. cerevisiae. Over-expression of xylulokinase encoding XKS1 stimulated growth on D-xylulose in a S. cerevisiae strain to about 20% of the growth rate on glucose and deletion of the gene prevented growth on D-xylulose and D-xylulose metabolism. We have partially purified the xylulokinase and characterised its kinetic properties. It is reversible and will also accept D-ribulose as a substrate. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：39 / 43

页数：5

共 23 条

[1] Cloning of a second gene encoding 6-phosphofructo-2-kinase in yeast, and characterization of mutant strains without fructose-2,6-bisphosphate [J].