Enhanced glycerol production in Shochu yeast by heat-shock treatment is due to prolonged transcription of GPD1

被引：18

作者：

Kajiwara, Y ^{[1
]}

Ogawa, K ^{[1
]}

Takashita, H ^{[1
]}

Omori, T ^{[1
]}

机构：

[1] Sanwa Shurui Co Ltd, Res Lab, Oita 8790495, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2000年 / 90卷 / 01期

关键词：

heat-shock; Saccharomyces cerevisiae; glycerol;

D O I：

10.1016/S1389-1723(00)80046-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Enhancement of glycerol production in Shochu yeast, which was induced by heat-shock treatment, was studied. Although heat-shock treatment (45 degrees C, 1 h) caused a transient delay in cell growth, the amount of glycerol produced by heat-shock-treated cells was 20% higher than that by control cells. During the glycerol-production phase, the NAD(+)-dependent glycerol-3-phosphate dehydrogenase (GPDH) activity of heat-shock-treated cells was much higher than that of control cells, suggesting that a higher GPDH activity enhances glycerol production. The level of NAD(+)-dependent glycerol dehydrogenase (GDH) activity was almost the same between heat-shock-treated cells and control cells. The results of Northern blot analysis of GPD genes (GPD1 and GPD2) encoding the GPDH enzyme showed that the transcription of GPD genes was not affected by heat-shock treatment but the period of intensive transcription of GPD1 was prolonged.

引用

页码：121 / 123

页数：3

共 15 条

[1]

GPD1, WHICH ENCODES GLYCEROL-3-PHOSPHATE DEHYDROGENASE, IS ESSENTIAL FOR GROWTH UNDER OSMOTIC-STRESS IN SACCHAROMYCES-CEREVISIAE, AND ITS EXPRESSION IS REGULATED BY THE HIGH-OSMOLARITY GLYCEROL RESPONSE PATHWAY [J].