Detection of Enteroaggregative Escherichia coli by Loop-Mediated Isothermal Amplification

被引：10

作者：

Yokoyama, Eiji ^{[1
]}

Uchimura, Masako ^{[1
]}

Ito, Kenitiro ^{[2
]}

机构：

[1] Chiba Prefectural Inst Publ Hlth, Div Bacteriol, Chuo Ku, Chiba 2608715, Japan

[2] Natl Inst Infect Dis, Infect Dis Surveillance Ctr, Tokyo 2080011, Japan

来源：

JOURNAL OF FOOD PROTECTION | 2010年 / 73卷 / 06期

关键词：

VIRULENCE CHARACTERISTICS; MOLECULAR EPIDEMIOLOGY; DIARRHEA; TRAVELERS; PREVALENCE; COUNTRIES; CHILDREN;

D O I：

10.4315/0362-028X-73.6.1064

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel gene amplification method, loop-mediated isothermal amplification (LAMP), has been recently developed as a rapid, specific diagnostic method for various infectious diseases. We have investigated whether LAMP can be used to detect small numbers of enteroaggregative Escherichia colt (EAEC) cells contaminated in food samples. Primers for LAMP reaction were designed with EAEC aggR gene sequences (available in GenBank). LAMP specificity with these primers was the same as that of PCR in a study of 37 EAEC and 42 non-EAEC bacterial strains. The sensitivity of the LAMP method was better than that of PCR in a study of serially diluted EAEC cells. The LAMP method was significantly more effective than was PCR in detecting EAEC-contaminated food samples (Fisher's exact test, P < 0.05). Therefore, the LAMP method described here should be useful for detecting small numbers of EAEC cells in food samples.

引用

页码：1064 / 1072

页数：9

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