DNA-based carbohydrate biochips: A platform for surface glyco-engineering

被引：130

作者：

Chevolot, Yann

Bouillon, Camille

Vidal, Sebastien

Morvan, Francois

Meyer, Albert

Cloarec, Jean-Pierre

Jochum, Anne

Praly, Jean-Pierre

Vasseur, Jean-Jacques

Souteyrand, Eliane

机构：

[1] Ecole Cent Lyon, CNRS, LEOM UMR 5512, F-69134 Ecully, France

[2] Univ Montpellier 2, CNRS, LCOBS UMR 5625, F-34095 Montpellier 5, France

[3] Univ Lyon 1, CNRS, LCO2 UMR 5246, F-69622 Villeurbanne, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 14期

关键词：

automated synthesis; biosensors; carbohydrates; click chemistry; microarrays;

D O I：

10.1002/anie.200604955

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

When the chips are down: A glycoarray consisting of oligonucleotide/ carbohydrate conjugates hybridized onto a DNA chip can be used for the immobilization of oligosaccharides at extremely low concentrations (1 μM). With lectin, the lower detection limit of the device was 2-20 nM. The relative surface density of the conjugates was monitored by Cy3 fluorescence. (Figure Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：2398 / 2402

页数：5

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