Extraction and fractionation of alkylresorcinols from triticale bran by two-step supercritical carbon dioxide

Bran from triticale cultivars Ultima and Pronghorn and Canadian Prime Spring (CPS) wheat were fractionated using a two-step sequential supercritical carbon dioxide (SC-CO2) extraction technique with and without ethanol to selectively extract and enrich high-value alkylresorcinols (ARs) Pure SC-CO2 (70 degrees C; flow rate 25 g/min. pressure 35 MPa), was used for the first extriction step The non-polar lipids yield ranged from 3 08 to 3 26 g/100 g and they were composed mainly of C18.2 (33-41 g/100 g), C18 1 (15-21 g/100 g), C16 0 (15-19 g/100 g), and C18:3 (4-5 g/100 g) fatty acids. The second extraction step. performed on the bran remaining after the first extraction, was carried out with SC-CO2 plus 0 5 mL/min ethanol (70 degrees C, flow rate 5 g/mm, pressure 35 MPa) The resulting total polar lipid yield of bran samples ranged between 0 62 and 0 86 g/100 g and the main Compounds present in the polar lipids were ARs The total AR contents of triticale cv Ultima, wheat, and triticale cv Pronghorn were 70, 68 and 36 mg/100 g, respectively Saturated and unsaturated AR homologues of C15 0, C 17 0, C19 0, C19:1, C21.0, C21.1, C23 0 and C25 0 were detected in all samples. Over 98% of the triticale ARs and over 95% of the wheat ARs were extracted under the SC-CO2 conditions used. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.