A basal transcription factor that activates or represses transcription

被引：132

作者：

Willy, PJ

Kobayashi, R

Kadonaga, JT ^{[1
]}

机构：

[1] Univ Calif San Diego, Mol Biol Sect, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Ctr Mol Genet, La Jolla, CA 92093 USA

[3] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

来源：

SCIENCE | 2000年 / 290卷 / 5493期

关键词：

D O I：

10.1126/science.290.5493.982

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have identified an activity that is required for transcription of downstream promoter element (DPE)-containing core promoters in vitro. The purified factor was found to be the Drosophila homolog of the transcriptional repressor known as NC2 or Dr1-Drap1. Purified recombinant dNC2 activates DPE-driven promoters and represses TATA-driven promoters. A mutant version of dNC2 can activate DPE promoters but is unable to repress TATA promoters. Thus, the activation and repression functions are distinct. These studies reveal that NC2 (Dr1-Drap1) is a bifunctional basal transcription factor that differentially regulates gene transcription through DPE or TATA box motifs.

引用

页码：982 / 984

页数：3

共 20 条

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