Cloning whole bacterial genomes in yeast

被引：108

作者：

Benders, Gwynedd A. ^{[1
]}

Noskov, Vladimir N. ^{[2
]}

Denisova, Evgeniya A. ^{[2
]}

Lartigue, Carole ^{[2
]}

Gibson, Daniel G. ^{[2
]}

Assad-Garcia, Nacyra ^{[2
]}

Chuang, Ray-Yuan ^{[2
]}

Carrera, William ^{[2
]}

Moodie, Monzia ^{[2
]}

Algire, Mikkel A. ^{[2
]}

Phan, Quang ^{[2
]}

Alperovich, Nina ^{[2
]}

Vashee, Sanjay ^{[2
]}

Merryman, Chuck ^{[2
]}

Venter, J. Craig ^{[1
,2
]}

Smith, Hamilton O. ^{[1
]}

Glass, John I. ^{[2
]}

Hutchison, Clyde A., III ^{[1
]}

机构：

[1] J Craig Venter Inst, Synthet Biol & Bioenergy Grp, San Diego, CA 92121 USA

[2] J Craig Venter Inst, Synthet Biol & Bioenergy Grp, Rockville, MD 20850 USA

来源：

NUCLEIC ACIDS RESEARCH | 2010年 / 38卷 / 08期

关键词：

TRANSFORMATION-ASSOCIATED RECOMBINATION; ARTIFICIAL CHROMOSOME; SELECTIVE ISOLATION; DNA-MOLECULES; EFFICIENT MANIPULATION; ESCHERICHIA-COLI; COMPLEX GENOMES; GENE; REPLICATION; MYCOIDES;

D O I：

10.1093/nar/gkq119

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional characterization. Here we report the cloning of whole bacterial genomes in the yeast Saccharomyces cerevisiae as single-DNA molecules. We cloned the genomes of Mycoplasma genitalium (0.6 Mb), M. pneumoniae (0.8 Mb) and M. mycoides subspecies capri (1.1 Mb) as yeast circular centromeric plasmids. These genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation.

引用

页码：2558 / 2569

页数：12

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