Crystallization and preliminary X-ray crystallographic analysis of plant ferritin from Glycine max

被引：8

作者：

Masuda, T

Mikami, B

Goto, F

Yoshihara, T

Utsumi, S

机构：

[1] Cent Res Inst Elect Power Ind, Dept Biosci, Chiba 2701194, Japan

[2] Kyoto Univ, Lab Food Qual Deisgn & Dev, Grad Sch Agr, Kyoto 6110011, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2003年 / 1645卷 / 01期

关键词：

plant ferritin; crystallization; Glycine max;

D O I：

10.1016/S1570-9639(02)00523-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The iron storage protein ferritin from soybean (Glycine max) was expressed in E. coli and crystallized using the hanging drop vapor diffusion method with sodium tartrate as the precipitant. The crystals belong to the tetragonal 14,22 space group, with unit cell parameters a = b = 324.0, c = 182.7 Angstrom. The diffraction data were collected up to a resolution of 3.0 Angstrom with a multi-wire area detector. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：113 / 115

页数：3

共 23 条

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[2]

Briat J. F., 1995, Iron nutrition in soils and plants. Proceedings of the Seventh International Symposium on Iron Nutrition and Interactions in Plants, Zaragoza, Spain, 27 June-2 July 1993., P265

[3] The 2.6 Å resolution structure of Rhodobacter capsulatus bacterioferritin with metal-free dinuclear site and heme iron in a crystallographic 'special position' [J].