RAPD PCR-based differentiation of Xanthomonas campestris pv. phaseoli and Xanthomonas campestris pv. phaseoli var. fuscans

被引:38
作者
Birch, PRJ
Hyman, LJ
Taylor, R
Opio, AF
Bragard, C
Toth, IK [1 ]
机构
[1] Scottish Crop Res Inst, Dept Fungal & Bacterial Plant Pathol, Dundee DD2 5DA, Scotland
[2] Hort & Food Res Inst New Zealand Ltd, Mt Albert Res Ctr, Auckland, New Zealand
[3] Kawanda Res Stn, Kampala, Uganda
关键词
RAPD PCR; Xanthomonas campestris pv. phaseoli; common bacterial blight; fuscous blight;
D O I
10.1023/A:1008698726790
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A RAPD PCR-based method was used to differentiate between isolates of Xanthomonas campestris pv. phaseoli and Xanthomonas campestris pv. phaseoli var. fuscans. Using random primer OP-G11, a single, high intensity band of 820 bp was amplified from DNAs of all X. c. pv. phaseoli var. fuscans isolates, while multiple amplification products of varying sizes were generated from X. c. pv. phaseoli DNAs. Whereas RAPD PCR differentiation gave an unambiguous result in under 4 h, standard differentiation by recording the production of a brown pigment by X. c. pv. phaseoli var. fuscans isolates took up to 7 days and showed variation both between isolates and between media. The unequivocal nature of the RAPD PCR method was demonstrated when isolate 408, originally classified as X. c. pv, phaseoli var. fuscans, failed to produce the 820 bp band typical of X. c. pv. phaseoli var. fuscans isolates, and after also failing to produce a brown pigment, was re-classified as X. c. pv. phaseoli.
引用
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页码:809 / 814
页数:6
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