The cell cycle inhibitor p21CIP is phosphorylated by cyclin A-CDK2 complexes

被引：14

作者：

Jaumot, M

Estañol, JM

Casanovas, O

Graña, X

Agell, N

Bachs, O

机构：

[1] Univ Barcelona, Fac Med, Dept Cell Biol, Barcelona 08036, Spain

[2] Temple Univ, Sch Med, Dept Biochem, Fels Inst Canc Res & Mol Biol, Philadelphia, PA 19140 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 241卷 / 02期

关键词：

D O I：

10.1006/bbrc.1997.7787

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here experimental evidence indicating that the p21(CIP), the universal inhibitor of cyclin-dependent protein kinases, general inhibitorCDKs is a substrate oof cyclin A-cdk2. The evidence comes from phosphorylation experiments in which the endogenous p21CIP present in using the original cyclin A-cdk2 complexes immunoprecipitated from HeLa cells extracts can be phosphorylated by the cdk2 of the same complexes. In vitro experiments showing that reconstituted GST cyclin A-GSTcdk2 complexes from phosphorylate recombinant GSTp21(CIP) confirms that p21CIP is a cyclin A-cdk2 substrate. (C) 1997 Academic Press.

引用

页码：434 / 438

页数：5

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