Intragenic suppressors of induction-deficient TetR mutants: Localization and potential mechanism of action

被引：5

作者：

Biburger, M ^{[1
]}

Berens, C ^{[1
]}

Lederer, T ^{[1
]}

Krec, T ^{[1
]}

Hillen, W ^{[1
]}

机构：

[1] Univ Erlangen Nurnberg, Inst Mikrobiol Biochem & Genet, Lehrstuhl Mikrobiol, D-91058 Erlangen, Germany

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 03期

关键词：

D O I：

10.1128/JB.180.3.737-741.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Eight Tn10 Tet repressor mutants with an induction-deficient phenotype and with primary mutations located at or close to the dimer interface were mutagenized and screened for inducibility in the presence of tetracycline, The second-site suppressors with wild-type-like operator binding activity that were obtained act, except for one, at a distance, suggesting that they contribute to conformational changes in the Tet repressor. Many of these long-range suppressors occur along the dimer interface, indicating that interactions between the monomers play an important role in Tet repressor induction.

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页码：737 / 741

页数：5

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