Biochemical effects of molecular crowding

被引：164

作者：

Chebotareva, NA ^{[1
]}

Kurganov, BI ^{[1
]}

Livanova, NB ^{[1
]}

机构：

[1] Russian Acad Sci, Bach Inst Biochem, Moscow 119071, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2004年 / 69卷 / 11期

关键词：

excluded volume; crowding; protein folding; association; denaturation; aggregation;

D O I：

10.1007/s10541-005-0070-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Cell cytoplasm contains high concentrations of high-molecular-weight components that occupy a substantial part of the volume of the medium (crowding conditions). The effect of crowding on biochemical processes proceeding in the cell (conformational transitions of biomacromolecules, assembling of macromolecular structures, protein folding, protein aggregation, etc.) is discussed in this review. The excluded volume concept, which allows the effects of crowding on biochemical reactions to be quantitatively described, is considered. Experimental data demonstrating the biochemical effects of crowding imitated by both low-molecular-weight and high-molecular-weight crowding agents are summarized.

引用

页码：1239 / +

页数：14

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