Seroconversion, neutralising antibodies and protection in bluetongue serotype 8 vaccinated sheep

被引：60

作者：

Oura, C. A. L. ^{[1
]}

Wood, J. L. N. ^{[2
]}

Sanders, A. J. ^{[1
]}

Bin-Tarif, A. ^{[1
]}

Henstock, M. ^{[1
]}

Edwards, L. ^{[1
]}

Floyd, T. ^{[2
]}

Simmons, H. ^{[3
]}

Batten, C. A. ^{[1
]}

机构：

[1] AFRC, Inst Anim Hlth, Pirbright Lab, Woking GU24 0NF, Surrey, England

[2] Univ Cambridge, Dept Vet Med, Cambridge Infect Dis Consortium, Cambridge CB3 0ES, England

[3] VLA Weybridge, Addlestone KT15 3NB, Surrey, England

来源：

VACCINE | 2009年 / 27卷 / 52期

关键词：

Bluetongue serotype 8; Inactivated vaccine; Sheep; ELISA; PCR DETECTION METHODS; INACTIVATED VACCINE; UNITED-KINGDOM; EVALUATE ELISA; VIRUS; CHALLENGE; EUROPE;

D O I：

10.1016/j.vaccine.2009.09.070

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Bluetongue virus serotype 8 (BTV-8) has caused a major outbreak of disease in cattle and sheep in several countries across northern and western Europe from 2006 to 2008. In 2008 the European Union instigated a mass-vaccination programme in affected countries using whole virus inactivated vaccines. We evaluated vaccinal responses in sheep and the ability of the vaccine to protect against experimental challenge. Sheep vaccinated 10 months previously under field conditions were challenged with BTV-8. One of 7 vaccinated sheep became infected, as evidenced by detection of viral RNA by real-time RT-PCR and by virus isolation. The remaining 6 sheep appeared fully protected from virus replication. None of the vaccinated sheep showed clinical signs of BTV and there was a good correlation between the presence of neutralising antibodies on challenge and protection. Commercially available ELISAs were evaluated for their ability to detect antibodies in sheep vaccinated on a single occasion. The sandwich (double antigen) ELISA assays were found to be more sensitive at detecting antibodies in vaccinated sheep than the competitive ELISAs. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：7326 / 7330

页数：5

共 17 条

[1] Bluetongue virus: European Community inter-laboratory comparison tests to evaluate ELISA and RT-PCR detection methods [J].

Batten, C. A. ;

Bachanek-Bankowska, K. ;

Bin-Tarif, A. ;

Kgosana, L. ;

Swain, A. J. ;

Corteyn, M. ;

Darpel, K. ;

Mellor, P. S. ;

Elliott, H. G. ;

Oura, C. A. L. .

VETERINARY MICROBIOLOGY, 2008, 129 (1-2) :80-88

[2] Bluetongue virus: European Community proficiency test (2007) to evaluate ELISA and RT-PCR detection methods with special reference to pooling of samples [J].

Batten, C. A. ;

Sanders, A. J. ;

Bachanek-Bankowska, K. ;

Bin-Tarif, A. ;

Oura, C. A. L. .

VETERINARY MICROBIOLOGY, 2009, 135 (3-4) :380-383

[3] Why were there no outbreaks of bluetongue in the UK during 2008? [J].

Burgin, L. ;

Gloster, J. ;

Mellor, P. S. .

VETERINARY RECORD, 2009, 164 (13) :384-387

[4] Efficacy of three inactivated vaccines against bluetongue virus serotype 8 in sheep [J].

Eschbaumer, Michael ;

Hoffmann, Bernd ;

Koenig, Patricia ;

Teifke, Jens P. ;

Gethmann, Joern M. ;

Conraths, Franz J. ;

Probst, Carolina ;

Mettenleiter, Thomas C. ;

Beer, Martin .

VACCINE, 2009, 27 (31) :4169-4175

[5] Bluetongue in the United Kingdom and northern Europe in 2007 and key issues for 2008 [J].

Gloster, J. ;

Burgin, L. ;

Witham, C. ;

Athanassiadou, M. ;

Mellor, P. S. .

VETERINARY RECORD, 2008, 162 (10) :298-302

[6] Will bluetongue come on the wind to the United Kingdom in 2007? [J].

Gloster, J. ;

Mellor, P. S. ;

Burgin, L. ;

Ganders, C. ;

Carpenter, S. .

VETERINARY RECORD, 2007, 160 (13) :422-426

[7]

HAIG D. A., 1956, ONDER STEPOORT JOUR VET RES, V27, P171

[8] Protective duration of immunity of an inactivated bluetongue (BTV) serotype 2 vaccine against a virulent BTV serotype 2 challenge in sheep [J].

Hamers, C. ;

Rehbein, S. ;

Hudelet, P. ;

Blanchet, M. ;

Lapostolle, B. ;

Cariou, C. ;

Duboeuf, M. ;

Goutebroze, S. .

VACCINE, 2009, 27 (21) :2789-2793

[9] Bluetongue virus serotype 8 reemergence in Germany, 2007 and 2008 [J].

Hoffmann, Bernd ;

Sasserath, Michael ;

Thalheim, Sabine ;

Bunzenthal, Claudia ;

Strebelow, Guenter ;

Beer, Martin .

EMERGING INFECTIOUS DISEASES, 2008, 14 (09) :1421-1423

[10]

HUISMANS H, 1981, ONDERSTEPOORT J VET, V48, P51

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