Study on highly sensitive potentiometric IgG immunosensor

被引:49
作者
Feng, CL [1 ]
Xu, YH
Song, LM
机构
[1] Liaoning Normal Univ, Dept Chem, Dalian 116029, Peoples R China
[2] Dalian Municipal Design & Res Inst Environm Sci, Dalian 116023, Peoples R China
基金
中国国家自然科学基金;
关键词
Number:; -; Acronym:; Sponsor: Natural Science Foundation of Liaoning Province; Sponsor: National Natural Science Foundation of China;
D O I
10.1016/S0925-4005(00)00382-8
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel potentiometric immunosensor for detection of immunogloblin G (IgG) has been developed by means of covalent immobilization of anti-immunogloblin G on a silver (Ag) electrode. The detection is based on the change in the electric potential before and after the antigen-antibody reaction. Tests relating to the detection of IgG demonstrate that the immunosensor exhibits a high level of sensitivity, stability and reproducibility, the linear response range was 0.2-1.2 ng/ml for IgG solution and the lifetime was more than 28 days. (C) 2000 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:190 / 192
页数:3
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