Differentiation-specific nuclear matrix proteins cross-linked to DNA by cis-diammine dichloroplatinum

DNA-protein cross-linkages were performed in intact undifferentiated and differentiated HL60 cells by the action of cis-diammine dichloroplatinum. Total nuclear matrix proteins and DNA cross-linked nuclear matrix proteins were resolved by two dimensional gel electrophoresis. The comparison of the electrophoretic patterns allowed the identification of st set of differentiation-induced nuclear matrix proteins cross-linked to DNA. One of these proteins binds cloned histone SAR sequences. Our results outline an experimental strategy for isolating and characterizing nuclear matrix components that may play a fundamental role in the overall control and coordination of gene expression during differentiation. (C) 1998 Academic Press.