Optimization and modifications of aptamers selected from live cancer cell lines

被引：223

作者：

Shangguan, Dihua

Tang, Zhiwen

Mallikaratchy, Prabodika

Xiao, Zeyu

Tan, Weihong ^{[1
]}

机构：

[1] Univ Florida, Dept Chem, Gainesville, FL 32611 USA

[2] Univ Florida, Shands Canc Ctr, UF Genet Inst, Gainesville, FL 32611 USA

[3] Univ Florida, McKnight Brain Inst, Ctr Res Bionano Interface, Gainesville, FL 32611 USA

来源：

CHEMBIOCHEM | 2007年 / 8卷 / 06期

关键词：

aptamers; cell recognition; DNA structures; nuclease resistance;

D O I：

10.1002/cbic.200600532

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Figure Presented) To cut a long story short. Single-stranded DNA aptamers that can recognize cancer cells specifically were optimized and modified. The modifications gave rise to aptamers that were much smaller in size (see scheme) and more stable in fetal bovine serum. This stability makes it possible to apply aptamers to in vivo applications, such as diagnosis and therapy. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：603 / 606

页数：4

共 21 条

[1] Natural and engineered nucleic acids as tools to explore biology [J].