Detection of differentially expressed genes in breast cancer with cDNA-array hybridization

被引：4

作者：

Ahr, A ^{[1
]}

Holtrich, U ^{[1
]}

Karn, T ^{[1
]}

Solbach, C ^{[1
]}

Gätje, R ^{[1
]}

Scharl, A ^{[1
]}

Strebhardt, K ^{[1
]}

Kaufmann, M ^{[1
]}

机构：

[1] Univ Frankfurt, Frauenklin, Bereich Mol Gynakol & Geburtshilfe, D-60590 Frankfurt, Germany

来源：

GEBURTSHILFE UND FRAUENHEILKUNDE | 2000年 / 60卷 / 08期

关键词：

D O I：

10.1055/s-2000-7426

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: Differentially expressed genes are potential prognostic factors in human breast cancer. cDNA-array hybridization permits rapids simultaneous analysis of differentially expressed genes. Methods: We studied the mRNA expression of 588 genes with cDNA-array analysis in 16 primary breast carcinomas (T1-4, N0-1, M0-1). Results: Approximately 1.5% of the analyzed genes showed significant differences in expression. Differentially expressed genes included c-erbB2 (HER2/neu), metalloproteinases, and newly identified transcripts. The expression of most genes did not seen to be altered significantly. Conclusion: The cDNA-array hybridization technique is a powerful tool to identify differentially expressed genes in breast cancers. These gene profiles will be studied for their prognostic significance.

引用

页码：412 / 417

页数：6

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