Stoichiometric studies of tannin-protein co-precipitation

被引:63
作者
Kawamoto, H
Nakatsubo, F
Murakami, K
机构
[1] Faculty of Agriculture, Kyoto University, Sakyo-ku
关键词
tannin; hydrolysable tannin; galloylglucose; tannin-protein co-precipitation; stoichiometry; complexation; relative affinity; mechanism;
D O I
10.1016/0031-9422(95)00728-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Co-precipitation of a series of galloylglucoses (hydrolysable tannins) with bovine serum albumin (BSA) was studied stoichiometrically by analysing both galloylglucoses and BSA in the precipitates using HPLC. BSA-precipitating ability increased mainly with an increase in the number of galloyl groups in a galloylglucose molecule but was also affected by the position of the galloyl group (penta- > tetra- > 2,3,6-tri- > 2,3,4-tri- much greater than di- much greater than monogalloylglucose). The precipitated BSA increased linearly with an increase in the number of galloyl groups bound to a BSA molecule. BSA-precipitating abilities of the galloylglucoses were closely related to their relative affinities for BSA. These results suggest a two-stage mechanism: initial complexation of galloylglucose with BSA and subsequent precipitation, as a mechanism of the co-precipitation.
引用
收藏
页码:1427 / 1431
页数:5
相关论文
共 18 条
[1]  
Bate-Smith E., 1954, Food, V23, P124
[2]   ANTIFUNGAL EFFECTS OF COCOA TANNIN ON THE WITCHES BROOM PATHOGEN CRINIPELLIS-PERNICIOSA [J].
BROWNLEE, HE ;
MCEUEN, AR ;
HEDGER, J ;
SCOTT, IM .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1990, 36 (01) :39-48
[4]   INHIBITION OF WOOD-ROTTING FUNGI BY ELLAGITANNINS IN HEARTWOOD OF QUERCUS-ALBA [J].
HART, JH ;
HILLIS, WE .
PHYTOPATHOLOGY, 1972, 62 (06) :620-&
[5]   POLYPHENOL-PROTEIN INTERACTIONS [J].
HASLAM, E .
BIOCHEMICAL JOURNAL, 1974, 139 (01) :285-288
[6]  
HASLAM E, 1989, PLANTA MED, P1
[7]  
HASLAM E, 1989, PLANT POLYPHENOLS, P156
[8]  
KAWAMOTO H, 1991, MOKUZAI GAKKAISHI, V37, P741
[9]   SYNTHESIS OF CONDENSED TANNIN DERIVATIVES AND THEIR PROTEIN - PRECIPITATING CAPACITY [J].
KAWAMOTO, H ;
NAKATSUBO, F ;
MURAKAMI, K .
JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY, 1990, 10 (01) :59-74
[10]   RELATIONSHIP BETWEEN THE B-RING HYDROXYLATION PATTERN OF CONDENSED TANNINS AND THEIR PROTEIN-PRECIPITATING CAPACITY [J].
KAWAMOTO, H ;
NAKATSUBO, F ;
MURAKAMI, K .
JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY, 1990, 10 (03) :401-409