Nanofabricated quartz cylinders for angular trapping: DNA supercoiling torque detection

被引：143

作者：

Deufel, Christopher ^{[1
]}

Forth, Scott ^{[1
]}

Simmons, Chad R. ^{[1
]}

Dejgosha, Siavash ^{[1
]}

Wang, Michelle D. ^{[1
]}

机构：

[1] Cornell Univ, Dept Phys, Atom & Solid State Phys Lab, Ithaca, NY 14853 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 03期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1038/nmeth1013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We designed and created nanofabricated quartz cylinders well suited for torque application and detection in an angular optical trap. We made the cylinder axis perpendicular to the extraordinary axis of the quartz crystal and chemically functionalized only one end of each cylinder for attachment to a DNA molecule. We directly measured the torque on a single DNA molecule as it underwent a phase transition from B-form to supercoiled P-form.

引用

页码：223 / 225

页数：3

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