Role for the disulfide-bonded region of human immunodeficiency virus type 1 gp41 in receptor-triggered activation of membrane fusion function

被引：10

作者：

Bellamy-McIntyre, Anna K. ^{[1
,2
]}

Baer, Severine ^{[3
,4
]}

Ludlow, Louise ^{[1
]}

Drummer, Heidi E. ^{[1
,2
,5
]}

Poumbourios, Pantelis ^{[1
,2
]}

机构：

[1] Macfarlane Burnet Inst Med Res & Publ Hlth, Melbourne, Vic 3004, Australia

[2] Monash Univ, Dept Microbiol, Melbourne, Vic 3168, Australia

[3] Deutsch Krebsforschungszentrum, Program Infect & Canc, Abt F010, Heidelberg, Germany

[4] Deutsch Krebsforschungszentrum, INSERM, U701, Heidelberg, Germany

[5] Univ Melbourne, Dept Microbiol & Immunol, Melbourne, Vic 3010, Australia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 394卷 / 04期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

HIV-1; Receptor; gp41; Disulfide-bonded region; Membrane fusion; TRANSMEMBRANE GLYCOPROTEIN; ENVELOPE GLYCOPROTEIN; ATOMIC-STRUCTURE; HIV-1; GP120; ECTODOMAIN; ISOMERIZATION; ARCHITECTURE; ASSOCIATION; STABILITY; ANTIBODY;

D O I：

10.1016/j.bbrc.2010.03.071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The conserved disulfide-bonded region (DSR) of the human immunodeficiency virus type 1 (HIV-1) fusion glycoprotein, gp41, mediates association with the receptor-binding glycoprotein, gp120. Interactions between gp120, CD4 and chemokine receptors activate the fusion activity of gp41. The introduction of W596L and W610F mutations to the DSR of HIV-1(QH1549.13) blocked viral entry and hemifusion without affecting gp120-gp41 association. The fusion defect correlated with inhibition of CD4-triggered gp41 pre-hairpin formation, consistent with the DSR mutations having decoupled receptor-induced conformational changes in gp120 from gp41 activation. Our data implicate the DSR in sensing conformational changes in the gp120-gp41 complex that lead to fusion activation. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：904 / 908

页数：5

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