Herbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats

被引:62
作者
Yan, Jingyu [1 ,2 ]
Xie, Guoxiang [3 ,4 ]
Liang, Chungeng [2 ]
Hu, Yiyang [1 ,2 ]
Zhao, Aihua [4 ]
Huang, Fengjie [4 ]
Hu, Ping [5 ]
Liu, Ping [1 ,2 ]
Jia, Wei [3 ,4 ]
Wang, Xiaoning [1 ,2 ]
机构
[1] Shanghai Univ Tradit Chinese Med, E Inst Shanghai Municipal Educ Commiss, Shanghai 201203, Peoples R China
[2] Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Inst Liver Dis, Shanghai 201203, Peoples R China
[3] Univ Hawaii, Ctr Canc, Honolulu, HI 96813 USA
[4] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Ctr Translat Med, Shanghai 200233, Peoples R China
[5] East China Univ Sci & Technol, Sch Chem & Mol Engn, Lab Funct Mat Chem, Shanghai 200237, Peoples R China
基金
上海市自然科学基金; 中国国家自然科学基金; 中国博士后科学基金;
关键词
CHEN-HAO-TANG; BILE-ACID METABOLISM; HEPATOCYTE APOPTOSIS; HEPATIC MITOCHONDRIA; T-CELLS; LIVER; INTERLEUKIN-10; EXPRESSION; MECHANISM; FIBROSIS;
D O I
10.1038/s41598-017-04536-5
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];
摘要
Cholestasis is a clinical disorder defined as an impairment of bile flow, and that leads to toxic bile acid (BA) accumulation in hepatocytes. Here, we investigated the hepatoprotective effect of Yinchenhaotang (YCHT), a well-known formulae for the treatment of jaundice and liver disorders, against the cholestasis using the alpha-naphthylisothiocyanate (ANIT)-induced cholestasis in male Wistar rats. ANIT feeding induced significant cholestasis with substantially increased intrahepatic retention of hydrophobic BAs. The dynamic changes of serum and liver BAs indicated that YCHT was able to attenuate ANIT-induced BA perturbation, which is consistent with the histopathological findings that YCHT significantly decreased the liver damage. YCHT treatment substantially reduced serum alanine aminotransferase (ALT), alkaline phosphatase (AST), total bilirubin (TBIL) and direct bilirubin (DBIL) with minimal bile duct damage in the ANIT treated rats. Elevated mRNA expression of liver IL-6, IL-17A, IL-17F, TGF-beta 1, alpha-SMA, TGR5, NTCP, OATP1a1, and ileum ASBT and decreased liver IL-10, FXR, CAR, VDR, BSEP, MRP2, MRP3, MRP4 was also observed in ANIT-induced cholestasis but were attenuated or normalized by YCHT. Our results demonstrated that the BA profiles were significantly altered with ANIT intervention and YCHT possesses the hepatoprotective potential against cholestatic liver injury induced by hepatotoxin such as ANIT.
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页数:13
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