Role of ERp57 in the signaling and transcriptional activity of STAT3 in a melanoma cell line

被引：43

作者：

Chichiarelli, Silvia ^{[1
]}

Gaucci, Elisa ^{[1
]}

Ferraro, Anna ^{[1
,2
]}

Grillo, Caterina ^{[1
]}

Altieri, Fabio ^{[1
,2
]}

Cocchiola, Rossana ^{[1
]}

Arcangeli, Valentina ^{[1
]}

Turano, Carlo ^{[1
,2
]}

Eufemi, Margherita ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dipartimento Sci Biochim, Fdn Cenci Bolognetti, Ist Pasteur, I-00185 Rome, Italy

[2] CNR, Ist Biol & Patol Mol, Rome, Italy

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2010年 / 494卷 / 02期

关键词：

STAT3; ERp57; Transcription regulation; STAT3-associated proteins; CRP; PROTEIN DISULFIDE-ISOMERASE; CROSS-LINKING; DNA; TRANSLOCATION; ACTIVATION; BINDING; CIS-DIAMMINEDICHLOROPLATINUM(II); IMMUNOGENICITY; CHAPERONE;

D O I：

10.1016/j.abb.2009.12.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Chromatin immunoprecipitation in M14 melanoma cells showed that the protein ERp57 (endoplasmic reticulum protein 57) binds to DNA in the proximity of STAT3 in a subset of STAT3-regulated genes. In the same cells, IL-6 induced a significant increase of the expression of one of these genes, i.e. CRP. Upon depletion of ERp57 by RNA interference. the phosphorylation of STAT3 on tyrosine 705 was decreased. and the IL-6-induced activation of CRP expression was completely suppressed. In vitro experiments showed that ERp57 is also required for the binding of STAT3 to its consensus sequence on DNA. Thus ERp57, previously shown to associate with STAT3 in the cytosol and in the nuclear STAT3-containing enhanceosome, is a necessary cofactor for the regulation of at least a subset of STAT3-dependent genes, probably intervening both at the site of STAT3 phosphorylation and at the nuclear level. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：178 / 183

页数：6

共 33 条

[1]

CIS-DIAMMINEDICHLOROPLATINUM(II) AND TRANS-DIAMMINEDICHLOROPLATINUM(II)-MEDIATED CROSS-LINKING OF CHROMOSOMAL NON-HISTONE PROTEINS TO DNA IN HELA-CELLS [J].