Chikusetsusaponin V inhibits inflammatory responses via NF-κB and MAPK signaling pathways in LPS-induced RAW 264.7 macrophages

被引：25

作者：

Wang, Ting ^{[1
,2
]}

Dai, Yanwen ^{[1
,3
]}

Dun, Yaoyan ^{[1
,2
]}

Zhang, Changcheng ^{[1
,2
]}

Wan, Jingzhi ^{[1
]}

Deng, Lili ^{[1
]}

Zhou, Zhiyong ^{[1
]}

Liu, Chaoqi ^{[4
]}

Yuan, Ding ^{[1
,2
]}

机构：

[1] China Three Gorges Univ, Coll Med Sci, Dept Pharmacol, Yichang 443002, Hubei, Peoples R China

[2] China Three Gorges Univ, Coll Med Sci, State Adm Tradit Chinese Med, Grade Pharmacol Lab Chinese Med 3, Yichang 443002, Hubei, Peoples R China

[3] China Three Gorges Univ, Coll Med Sci, Renhe Hosp, Dept Reprod Ctr, Yichang 443002, Hubei, Peoples R China

[4] China Three Gorges Univ, Coll Med Sci, Dept Mol Biol, Yichang 443002, Hubei, Peoples R China

来源：

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY | 2014年 / 36卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Chikusetsusaponin V; inflammatory; LPS; MAPK; NF-kappa BKW; AMELIORATES LUNG INFLAMMATION; TOLL-LIKE RECEPTORS; PANAX-JAPONICUS; MEDIATORS; SAPONINS; TLR4; MICE; SUPPRESSION; MECHANISMS; EXPRESSION;

D O I：

10.3109/08923973.2014.960088

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Excessive activation of macrophages is implicated in various inflammation resulted injuries. Saponins from Panax japonicus (SPJ) have been shown to possess anti-inflammatory activities. However, whether Chikusetsusaponin V (CsV), the most abundant component of SPJ, can exert anti-inflammatory activities is unknown. The present study was aimed to investigate the anti-inflammatory effects of CsV in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells and the underlying mechanisms. Our data showed that CsV dose-dependently inhibited NO, iNOS, TNF-alpha and IL-1 beta expressions in LPS-stimulated RAW264.7 cells. Increased protein levels of nuclear NF-kappa B and elevated phosphorylation levels of ERK and JNK in LPS-stimulated RAW 264.7 cells were also found downregulated by CsV treatment. Furthermore, the increase of CD14 and TLR4 mRNA expression due to LPS stimulation were significantly reversed by CsV treatment. These results suggested that CsV attenuated LPS-induced inflammatory responses partly via TLR4/CD14-mediated NF-kappa B and MAPK pathways.

引用

页码：404 / 411

页数：8

共 35 条

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