Determination of the sensitivity of a rapid Escherichia coli O157:H7 assay for testing 375-gram composite samples

被引：12

作者：

Tsai, WL

Miller, CE

Richter, ER

机构：

[1] Richter Int Inc, Columbus, OH 43201 USA

[2] Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 09期

关键词：

D O I：

10.1128/AEM.66.9.4149-4151.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Both 25-g single-size ground beef samples and 375-g composite ground beef samples were tested by a method combining an immunomagnetic separation (IMS) technique with a sandwich enzyme-linked immunosorbent assay (ELISA) system (IRIS-ELISA). The results demonstrated that IMS-ELISA could detect the target, Escherichia coli O157:H7, at the level of 10(-1) CFU/g of sample in either the 25- or 375-g sample size.

引用

页码：4149 / 4151

页数：3

共 22 条

[1]

ANDREWS WH, 1995, BACTERIOLOGICAL ANAL

[2]

[Anonymous], 1997, Morbidity and Mortality Weekly Report, V46, P777

[3] AN OUTBREAK OF DIARRHEA AND HEMOLYTIC UREMIC SYNDROME FROM ESCHERICHIA-COLI O157-H7 IN FRESH-PRESSED APPLE CIDER [J].