Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells

被引：126

作者：

Fu, Y

Rieg, G

Fonzi, WA

Belanger, PH

Edwards, JE

Filler, SG

机构：

[1] Univ Calif Los Angeles, Harbor Res & Educ Inst,St, Div Infect Dis, Dept Med,St Johns Cardiovasc Res Ctr, Torrance, CA 90502 USA

[2] Georgetown Univ, Dept Microbiol & Immunol, Washington, DC 20007 USA

[3] Univ Calif Los Angeles, Sch Med, Los Angeles, CA 90024 USA

来源：

INFECTION AND IMMUNITY | 1998年 / 66卷 / 04期

关键词：

D O I：

10.1128/IAI.66.4.1783-1786.1998

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae. These transformed organisms were screened for adherence to endothelial cells, and a highly adherent clone was identified. The adherence of this clone to endothelial cells was over 100-fold greater than that of control S. cerevisiae transformed with the empty plasmid. This clone also exhibited enhanced adherence to epithelial cells. The C. albicans gene contained within this clone was found to be ALS1. These results indicate that ALS1 may encode a candidal adhesin.

引用

页码：1783 / 1786

页数：4

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