Taqman real-time quantitative PCR for identification of western flower thrip (Frankliniella occidentalis) for plant quarantine

被引：39

作者：

Huang, K. S. ^{[2
]}

Lee, S. E. ^{[1
]}

Yeh, Y. ^{[2
]}

Shen, G. S. ^{[2
]}

Mei, E. ^{[1
]}

Chang, C. M. ^{[1
]}

机构：

[1] Chang Gung Univ, Dept Med Biotechnol & Lab Sci, Res Ctr Emerging Viral Infect, Tao Yuan 333, Taiwan

[2] Bur Anim & Plant Hlth Inspect & Quarantine, Council Agr, Hsinchu Branch, Tao Yuan 33758, Taiwan

来源：

BIOLOGY LETTERS | 2010年 / 6卷 / 04期

关键词：

Frankliniella occidentalis; real-time quantitative PCR; plant quarantine;

D O I：

10.1098/rsbl.2009.1060

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Western flower thrip (Frankliniella occidentalis) is a major global pest of agricultural products. It directly damages crops through feeding, oviposition activity or transmission of several plant viruses. We describe a Taqman real-time quantitative PCR detection system, which can rapidly identify F. occidentalis from thrips larvae to complement the traditional morphological identification. The data showed that our detection system targeted on the ribosomal RNA gene regions of F. occidentalis has high sensitivity and specificity. The rapid method can be used for on-site testing of samples at ports-of-entry in the future.

引用

页码：555 / 557

页数：3

共 8 条

[1] A molecular identification key for economically important thrips species (Thysanoptera: Thripidae) using direct sequencing and a PCR-RFLP-based approach [J].

Brunner, P. C. ;

Fleming, C. ;

Frey, J. E. .

AGRICULTURAL AND FOREST ENTOMOLOGY, 2002, 4 (02) :127-136

[2] The spread of the western flower thrips Frankliniella occidentalis (Pergande) [J].

Kirk, WDJ ;

Terry, LI .

AGRICULTURAL AND FOREST ENTOMOLOGY, 2003, 5 (04) :301-310

[3] Invasion biology of thrips [J].

Morse, JG ;

Hoddle, MS .

ANNUAL REVIEW OF ENTOMOLOGY, 2006, 51 :67-89

[4] Global status of tospovirus epidemics in diverse cropping systems: Successes achieved and challenges ahead [J].

Pappu, H. R. ;

Jones, R. A. C. ;

Jain, R. K. .

VIRUS RESEARCH, 2009, 141 (02) :219-236

[5]

SMITH IM, 1997, QUARANTINE PESTS EUR, P267

[6] Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region [J].

Toda, S ;

Komazaki, S .

BULLETIN OF ENTOMOLOGICAL RESEARCH, 2002, 92 (04) :359-363

[7] Development of a sequence-specific real-time PCR to the melon thrips Thrips palmi (Thysan., Thripidae) [J].

Walsh, K ;

Boonham, N ;

Barker, I ;

Collins, DW .

JOURNAL OF APPLIED ENTOMOLOGY, 2005, 129 (05) :272-279

[8] Tospovirus-thrips interactions [J].

Whitfield, AE ;

Ullman, DE ;

German, TL .

ANNUAL REVIEW OF PHYTOPATHOLOGY, 2005, 43 :459-489

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