Triplex targeting of a native gene in permeabilized intact cells: covalent modification of the gene for the chemokine receptor CCR5

被引：51

作者：

Belousov, ES ^{[1
]}

Afonina, IA ^{[1
]}

Kutyavin, IV ^{[1
]}

Gall, AA ^{[1
]}

Reed, MW ^{[1
]}

Gamper, HB ^{[1
]}

Wydro, RM ^{[1
]}

Meyer, RB ^{[1
]}

机构：

[1] Epoch Pharmaceut Inc, Bothell, WA 98021 USA

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 05期

关键词：

D O I：

10.1093/nar/26.5.1324

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A 12 nucleotide oligodeoxyribopurine tract in the gene for the chemokine receptor CCR5 has been targeted and covalently modified in intact cells by a 12mer tripler forming oligonucleotide (TFO) bearing a reactive group, A nitrogen mustard placed on the 5'-end of the purine motif TFO modified a guanine on the DNA target with high efficiency and selectivity, A new use of a guanine analog in these TFOs significantly enhanced tripler formation and efficiency of modification, as did the use of the tripler-stabilizing intercalator coralyne. This site-directed modification of a native chromosomal gene in intact human cells under conditions where many limitations of tripler formation have been partially addressed underscores the potential of this approach for gene control via site-directed mutagenesis.

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页码：1324 / 1328

页数：5

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