Development of a new method for analysis of Sudan dyes by pressurized CEC with amperometric detection

被引：45

作者：

Liu, Shaofeng ^{[1
]}

Zhang, Xue ^{[1
]}

Lin, Xucong ^{[1
]}

Wu, Xiaoping ^{[1
]}

Fu, Fengfu ^{[1
]}

Xie, Zenghong ^{[1
]}

机构：

[1] Fuzhou Univ, Dept Chem, Key Lab Anal & Detect Technol Food Safety, Minist Educ, Fuzhou 350002, Peoples R China

来源：

ELECTROPHORESIS | 2007年 / 28卷 / 11期

关键词：

amperometric detection; pressurized CEC; Sudan dyes;

D O I：

10.1002/elps.200600486

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A new analytical method, pressurized CEC (pCEC) with amperometric detection (AD) using 1.5 mu m RP nonporous silica packed columns has been developed for the rapid separation and determination of four Sudan dyes in hot chilli. The influence of several experimental parameters on the retention behavior has been investigated. The electrochemical oxidation of Sudans I-IV separated by pCEC can be reliably monitored with a carbon electrode at +0.95 V (vs. Ag/AgCl). Fast and efficient separation of the analytes was achieved within 7 min by pCEC under the optimum conditions with an ACN/water (95:5%) mobile phase containing formic acid (pH 4.3), 5% acetone and 0.002% triethylamine using a separation voltage of 12 W The detection limits for four Sudan dyes ranged from 8.0 x 10(-7) to 1.2 x 10(-6) mol/L. To evaluate the feasibility and reliability of this method, the proposed pCEC-AD method was further demonstrated with hot chilli samples spiked with Sudan dyes.

引用

页码：1696 / 1703

页数：8

共 27 条

[1]

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