Wnt/β-catenin signaling regulates cancer stem cells in lung cancer A549 cells

被引：380

作者：

Teng, Ying ^{[1
]}

Wang, Xiuwen ^{[1
]}

Wang, Yawei ^{[1
]}

Ma, Daoxin ^{[2
]}

机构：

[1] Shandong Univ, Ctr Canc, Dept Chemotherapy, Qilu Hosp, Jinan 250012, Peoples R China

[2] Shandong Univ, Ctr Canc, Dept Blood, Qilu Hosp, Jinan 250012, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 392卷 / 03期

关键词：

Signal transduction; Lung neoplasms; OCT-4; Target therapy; ACUTE MYELOID-LEUKEMIA; PROSPECTIVE IDENTIFICATION; BETA-CATENIN; OCT-4; WNT; EXPRESSION; NANOG;

D O I：

10.1016/j.bbrc.2010.01.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Wnt/beta-catenin signaling plays an important role not only in cancer, but also in cancer stem cells In this study, we found that beta-catenin and OCT-4 was highly expressed in cisplatin (DDP) selected A549 cells Stimulating A549 cells with lithium chloride (LiCl) resulted in accumulation of beta-catenin and up-regulation of a typical Writ target gene cyclin D1 This stimulation also significantly enhanced proliferation, clone formation, migration and drug resistance abilities in A549 cells Moreover. the Up-regulation of OCT-4, a stem cell marker, was observed through real-time PCR and Western blotting In a reverse approach, we inhibited Wilt signaling by knocking down the expression of beta-catenin using RNA interference technology This inhibition resulted in clown-regulation of the Wilt target gene cyclin D1 as well as the proliferation, clone formation, migration and drug resistance abilities Meanwhile, the expression of OCT-4 was reduced after the inhibition of Wnt/beta-catenin signaling Taken together. Our Study provides strong evidence that canonical Wnt signaling plays an important role in lung cancer stem cell properties, and it also regulates OCT-4, a lung cancer stem cell marker (C) 2010 Elsevier Inc All rights reserved

引用

页码：373 / 379

页数：7

共 31 条

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