Characterization of reference genes for quantitative real-time PCR analysis in various tissues of Salvia miltiorrhiza

被引：116

作者：

Yang, Yanfang ^{[1
,2
]}

Hou, Shuang ^{[1
,2
,4
]}

Cui, Guanghong ^{[3
]}

Chen, Shilin ^{[1
,2
]}

Wei, Jianhe ^{[1
,2
]}

Huang, Luqi ^{[3
]}

机构：

[1] Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China

[2] Peking Union Med Coll, Beijing 100193, Peoples R China

[3] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

[4] Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400715, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 01期

关键词：

Reference gene; Quantitative real-time PCR; Salvia miltiorrhiza; DXR; 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE; HOUSEKEEPING GENES; RT-PCR; INTERNAL CONTROL; MOLECULAR-CLONING; EXPRESSION; IDENTIFICATION; SELECTION; NORMALIZATION; TRANSCRIPTION;

D O I：

10.1007/s11033-009-9703-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five reference genes, 18S, EF1 alpha, alpha-Tubulin, Ubiquitin and Actin, from Salvia miltiorrhiza were analyzed as internal controls for gene expression profiling assay using quantitative real-time polymerase chain reaction (qRT-PCR). The five candidate genes were measured for their transcriptional level in seven tissues, including roots, stems, leaves, sepals, petals, stamens and pistils. Then they were ranked by the GeNorm tool. The results showed that Actin and Ubiquitin were the most stable whereas EF1 alpha and 18S did not favor normalization of qRT-PCR results in these tissues. Expression levels of the SmDXR gene were studied in parallel, with Actin and Ubiquitin both or each as reference in the seven tissues, and varying relative quantifications of the SmDXR gene in seven tissues. This study indicated that selection of the most stable genes plays an important role in gene expression profiling assays.

引用

页码：507 / 513

页数：7

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