Recombinant guinea pig TNF-α enhances antigen-specific type 1 T lymphocyte activation in guinea pig splenocytes

被引:34
作者
Cho, Hyosun [1 ]
McMurray, David N. [1 ]
机构
[1] Texas A&M Univ, Syst Hlth Sci Ctr, Dept Med Microbiol & Immunol, College Stn, TX 77843 USA
关键词
BCG; TNF-alpha IFN-gamma; guinea pig;
D O I
10.1016/j.tube.2005.12.001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
TNF-alpha is a principal pro-inflammatory cytokine which contributes to the activation of innate immunity and the transition to antigen-specific adaptive immunity in tuberculosis. Using recombinant guinea pig (rgp) TNF-alpha, the effect of TNF-alpha on lymphocyte activation was examined in unvaccinated and BCG-vaccinated guinea pigs. Splenocytes were stimulated with PPD or ConA, in the presence or absence of rgp TNF-alpha for 96h. Lymphocyte proliferation was measured using [H-3]thymidine uptake, and IL-12 p40 and IFN-gamma mRNA were analyzed using real-time PCR. rgpTNF-alpha atone was able to stimulate a significant degree of proliferation in splenocytes. The addition of rgpTNF-alpha to PPD-stimulated cells enhanced the proliferation of splenocytes from BCG-vaccinated guinea pigs. Furthermore, enhancement of proliferation by rgpTNF-alpha was found to be correlated with upregulation of the levels of Type 1 cytokine mRNA (IL-12p40 and IFN-gamma) in splenocyte cultures. This suggests that TNF-alpha plays an important role in the regulation of Type 1 T-cell-mediated immune responses in the guinea pig. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:87 / 93
页数:7
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