Locomotory phenotypes of human tumor cell lines and T lymphocytes in a three-dimensional collagen lattice

被引:32
作者
Niggemann, B [1 ]
Maaser, K [1 ]
Lu, HQ [1 ]
Kroczek, R [1 ]
Zanker, KS [1 ]
Friedl, P [1 ]
机构
[1] UNIV WURZBURG,DEPT DERMATOL,D-8700 WURZBURG,GERMANY
关键词
cell migration; tumor cells; T lymphocytes; collagen matrix; velocity;
D O I
10.1016/S0304-3835(97)00328-5
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Active cellular locomotion is a feature of such diverse cell types as lymphocytes and cancer cells. The locomotory phenotype of a cell should ultimately reflect the biochemical basis of different migratory strategies. We investigated the locomotory behavior of five epithelial cell lines and one non-epithelial human cell-line as well as human CD4(+) T lymphocytes in a three-dimensional collagen type I matrix using time-lapse video microscopy and computer assisted cell-tracking.. Migration velocity was up to 70 times lower in tumor cells (0.1-0.3 mu m/min) as compared to T lymphocytes (7-7.5 mu m/min), whereas the percentage of spontaneously active cells was up to twice as high in tumor cells (80-90%) in comparison to T lymphocytes (54%). Persistence, i.e. the degree of roaming, varied appreciably between the different cell types. In conclusion, velocity and persistence may describe distinct migration strategies in different cell types, i.e. discerning T cell migration from tumor cell invasion. (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:173 / 180
页数:8
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