Content of the HMG-17 chromosomal protein in porcine tissues

被引：4

作者：

Boumba, VA ^{[1
]}

Seferiadis, K

Vougiouklakis, T

机构：

[1] Univ Ioannina, Biol Chem Lab, GR-45110 Ioannina, Greece

[2] Univ Ioannina, Dept Forens Med & Toxicol, Sch Med, GR-45110 Ioannina, Greece

来源：

PROTEIN AND PEPTIDE LETTERS | 2004年 / 11卷 / 06期

关键词：

HMG-17; histone H1; quantification; ELISA; tissue distribution;

D O I：

10.2174/0929866043406193

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HMG-17 proteins are nucleosomal proteins, implicated in control of chromatin structure and transcription, in a way that has not yet been fully understood. In this report, quantification of HMG-17 in porcine tissues was performed, by ELISA, using previously produced and characterized specific rabbit anti-HMG-17 antibodies. Our results showed high levels of HMG-17 compared to the DNA and H1 content of the tissues (on the contrary to previous reports), and more specifically that there were 1.7 molecules of HMG-17 per molecule of histone H1.

引用

页码：515 / 519

页数：5

共 26 条

[1] ISOLATION BY A NEW METHOD AND SEQUENCE-ANALYSIS OF CHROMOSOMAL HMG-17 PROTEIN FROM PORCINE THYMUS [J].

BOUMBA, VA ;

TSOLAS, O ;

CHOLIPAPADOPOULOU, D ;

SEFERIADIS, K .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 303 (02) :436-442

[2] Rabbit anti-HMG-17 antibodies recognize similar epitopes on the HMG-17 molecule as lupus autoantibodies. Relation with histone H1 defined epitopes [J].

Boumba, VA ;

Seferiadis, K .

JOURNAL OF PEPTIDE SCIENCE, 2002, 8 (12) :683-694

[3]

BUCCI LR, 1984, J BIOL CHEM, V259, P8840

[4] STRUCTURAL FEATURES OF THE HMG CHROMOSOMAL-PROTEINS AND THEIR GENES [J].

BUSTIN, M ;

LEHN, DA ;

LANDSMAN, D .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1049 (03) :231-243

[5] AUTOANTIBODIES TO NUCLEOSOMAL PROTEINS - ANTIBODIES TO HMG-17 IN AUTOIMMUNE-DISEASES [J].

BUSTIN, M ;

REISCH, J ;

EINCK, L ;

KLIPPEL, JH .

SCIENCE, 1982, 215 (4537) :1245-1247

[6]

Bustin M, 1996, PROG NUCLEIC ACID RE, V54, P35, DOI 10.1016/S0079-6603(08)60360-8

[7] NUCLEOSOME CORE BINDING REGION OF CHROMOSOMAL PROTEIN HMG-17 ACTS AS AN INDEPENDENT FUNCTIONAL DOMAIN [J].

CRIPPA, MP ;

ALFONSO, PJ ;

BUSTIN, M .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 228 (02) :442-449

[8] CHARACTERIZATION OF A CHROMATIN FRACTION BEARING PULSE-LABELED RNA - .2. QUANTIFICATION OF HISTONES AND HIGH-MOBILITY-GROUP PROTEINS [J].

GABRIELLI, F ;

HANCOCK, R ;

FABER, AJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1981, 120 (02) :363-369

[9] NEW GROUP OF CHROMATIN-ASSOCIATED PROTEINS WITH A HIGH CONTENT OF ACIDIC AND BASIC AMINO-ACIDS [J].

GOODWIN, GH ;

SANDERS, C ;

JOHNS, EW .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1973, 38 (01) :14-19

[10] Dynamic relocation of chromosomal protein HMG-17 in the nucleus is dependent on transcriptional activity [J].

Hock, R ;

Wilde, F ;

Scheer, U ;

Bustin, M .

EMBO JOURNAL, 1998, 17 (23) :6992-7001

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