Unusual tazobactam-sensitive AmpC β-lactamase from two Escherichia coli isolates

被引:10
作者
Babini, GS
Danel, F
Munro, SD
Micklesen, PA
Livermore, DM
机构
[1] St Bartholomews & Royal London Sch Med & Dent, Dept Med Microbiol, London E1 2AD, England
[2] Stanford Univ Hosp, Med Ctr, Stanford, CA 94305 USA
关键词
D O I
10.1093/jac/41.1.115
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Two Escherichia coli isolates were studied. MIC patterns and hydrolysis assays suggested that they hyperproduced AmpC beta-lactamase, but synergy between ceftazidime and tazobactam was greater than between ceftazidime and Ro 48-1256, whereas the converse pattern is typical of AmpC hyperproducers. Studies with purified beta-lactamase from one of the isolates confirmed that tazobactam was a 100-fold stronger inhibitor than for the classical E. coli AmpC enzyme. Moreover, in contrast to typical AmpC types, the new enzyme had greater affinity for cephaloridine than for benzylpenicillin.
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页码:115 / 118
页数:4
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