Quantitative detection of apoptotic thymocytes in low-dose X-irradiated mice by an anti-single-stranded DNA antibody

被引:12
作者
Fujita, K
Kawarada, Y
Terada, K
Sugiyama, T
Ohyama, H
Yamada, T
机构
[1] Toho Univ, Sch Med, Dept Biol, Ota Ku, Tokyo 1438540, Japan
[2] Toho Univ, Grad Sch Sci, Div Biomol Sci, Funabashi, Chiba 2748511, Japan
[3] Akita Univ, Sch Med, Dept Biochem, Akita 0108545, Japan
[4] Natl Inst Radiol Sci, Div Radiobiol & Biodosimetry, Inage Ku, Chiba 2638555, Japan
关键词
D O I
10.1269/jrr.41.139
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The quantitative detection of apoptotic cells in low frequency in the thymus of mice irradiated with X-rays using an anti-single-stranded DNA antibody was explored. The antibody against single-stranded DNA (anti-ssDNA) was obtained with rabbits hyperimmunized with complexes of alkaline-denatured calf thymus DNA. AKR female mice were irradiated with 10 to 100 cGy or 4 Gy X-rays; thereafter, thymus sections were prepared at various times after irradiation. The detection and counting of apoptotic cells in the section were performed after histochemical staining using an anti-ssDNA antibody. The results demonstrate that, although sensitive and quantitative detection of apoptotic cells in irradiated thymus using the anti-ssDNA antibody is possible, the sensitivity is lower compared to that of in situ endlabeling methods, such as TUNEL or ISEL. The antibodies could also be used for rat thymus and spleen. In addition, an increase in positively stained cells by both methods was detected as early as 6 min after the irradiation of mice.
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页码:139 / 149
页数:11
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