Association of chlorophyll a/b-binding Pcb proteins with photosystems I and II in Prochlorothrix hollandica

被引:15
作者
Boichenko, Vladimir A. [1 ]
Pinevich, Alexander V.
Stadnichuk, Igor N.
机构
[1] Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142290, Russia
[2] St Petersburg State Univ, Inst Biol, St Petersburg 198904, Russia
[3] Russian Acad Sci, AN Bakh Biochem Inst, Moscow 117071, Russia
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2007年 / 1767卷 / 06期
关键词
photosystems I and II; Pcb protein; action spectra; antenna size;
D O I
10.1016/j.bbabio.2006.11.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Action spectra for photosystem II (PSII)-driven oxygen evolution and of photosystem I (PSI)-mediated H-2 photoproduction and photoinhibition of respiration were used to determine the participation of chlorophyll (Chl) a/b-binding Pcb proteins in the functions of pigment apparatus of Prochlorothrix hollandica. Comparison of the in situ action spectra with absorption spectra of PSII and PSI complexes isolated from the cyanobacterium Synechocystis 6803 revealed a shoulder at 650 nm that indicated presence of Chl b in the both photosystems of P hollandica. Fitting of two action spectra to absorption spectrum of the cells showed a chlorophyll ratio of 4:1 in favor of PSI. Effective antenna sizes estimated from photochemical cross-sections of the relevant photoreactions were found to be 192 +/- 28 and 139 +/- 15 chlorophyll molecules for the competent PSI and PSII reaction c:enters, respectively. The value for PSI is in a quite good agreement with previous electron microscopy data for isolated Pcb-PSI supercomplexes from P. hollandica that show a trimeric PSI core surrounded by a ring of 18 Pcb subunits. The antenna size of PSII implies that the PSII core dimers are associated with similar to 14 Pcb light-harvesting proteins, and form the largest known Pcb-PSII supercomplexes. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:801 / 806
页数:6
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