Natural latex, grass pollen, and weed pollen share IgE epitopes

被引:104
作者
Fuchs, T
Spitzauer, S
Vente, C
Hevler, J
Kapiotis, S
Rumpold, H
Kraft, D
Valenta, R
机构
[1] UNIV VIENNA,AKH,INST GEN & EXPT PATHOL,A-1090 VIENNA,AUSTRIA
[2] UNIV GOTTINGEN,DEPT DERMATOL,D-3400 GOTTINGEN,GERMANY
[3] UNIV VIENNA,AKH,INST MED & CHEM LAB DIAGNOST,VIENNA,AUSTRIA
[4] PHARMACIA & UPJOHN INC,FREIBURG,GERMANY
基金
奥地利科学基金会;
关键词
latex allergy; timothy grass pollen; mugwort pollen; ragweed pollen; IgE; cross-reactivity;
D O I
10.1016/S0091-6749(97)70249-5
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Because of the frequent use of natural latex products, IgE-mediated reactions to latex proteins represent an important health threat in industrialized countries, Although several latex allergens have been characterized and IgE cross-reactivities with allergens present in plant-derived food have been described, limited information is available regarding the presence of common IgE-binding components in latex and plant pollen, Methods: By using serum IgE from 56 individuals with latex allergy, the IgE-binding components in ammoniated latex milk and latex glove extracts were characterized by immunoblotting. The presence of cross-reactive IgE-binding components in the different latex extracts, extracts from mugwort, ragweed, timothy grass pollen, and recombinant birch pollen allergens (Bet v 1 and Bet v 2 [birch profilin]) was studied by immunoblot inhibitions and quantitative competition experiments. The involvement of carbohydrates in the constitution of cross-reactive IgE epitopes was studied by periodate treatment of extracts. Results: Although sera from certain individuals with latex allergy showed IgE reactivity with protein bands of different molecular weights in Western-blotted latex milk and glove extracts, both extracts contained common IgE epitopes. Although preincubation with recombinant Bet vl and Bet v 2 did not significantly inhibit IgE binding to latex proteins, weed and, in particular, timothy grass pollen extract strongly inhibited IgE binding to latex allergens, The cross-reactive IgE epitopes were sensitive to periodate treatment, Conclusions: Mugwort, ragweed, and timothy grass pollen share IgE epitopes with glycoprotein latex allergens, The presence of common epitopes might in part explain clinical symptoms in patients allergic to pollen on contact with latex.
引用
收藏
页码:356 / 364
页数:9
相关论文
共 56 条
[1]   CROSS-REACTING ALLERGENS IN NATURAL-RUBBER LATEX AND AVOCADO [J].
AHLROTH, M ;
ALENIUS, H ;
TURJANMAA, K ;
MAKINENKILJUNEN, S ;
REUNALA, T ;
PALOSUO, T .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1995, 96 (02) :167-173
[2]   SERUM REACTIVITIES TO LATEX PROTEINS (HEVEA-BRASILIENSIS) [J].
AKASAWA, A ;
HSIEH, LS ;
LIN, Y .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1995, 95 (06) :1196-1205
[3]   A novel acidic allergen, Hev b 5, in latex - Purification, cloning and characterization [J].
Akasawa, A ;
Hsieh, LS ;
Martin, BM ;
Liu, T ;
Lin, Y .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (41) :25389-25393
[4]   Comparison of latex-specific IgE binding among nonammoniated latex, ammoniated latex, and latex glove allergenic extracts by ELISA and immunoblot inhibition [J].
Akasawa, A ;
Hsieh, LS ;
Lin, Y .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1996, 97 (05) :1116-1120
[5]   IGE IMMUNE-RESPONSE TO RUBBER PROTEINS IN ADULT PATIENTS WITH LATEX ALLERGY [J].
ALENIUS, H ;
TURJANMAA, K ;
MAKINENKILJUNEN, S ;
REUNALA, T ;
PALOSUO, T .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1994, 93 (05) :859-863
[6]   Significance of rubber elongation factor as a latex allergen [J].
Alenius, H ;
Kalkkinen, N ;
Yip, E ;
Hasmin, H ;
Turjanmaa, K ;
MakinenKiljunen, S ;
Reunala, T ;
Palosuo, T .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1996, 109 (04) :362-368
[7]  
Alenius H, 1996, CLIN EXP ALLERGY, V26, P341, DOI 10.1046/j.1365-2222.1996.d01-312.x
[8]  
Alenius H, 1996, J IMMUNOL, V156, P1618
[9]   IGE-MEDIATED ANAPHYLACTOID REACTIONS TO RUBBER [J].
AXELSSON, JGK ;
JOHANSSON, SGO ;
WRANGSJO, K .
ALLERGY, 1987, 42 (01) :46-50
[10]   Cross-reactivity between the major allergen from olive pollen and unrelated glycoproteins: Evidence of an epitope in the glycan moiety of the allergen [J].
Batanero, E ;
Villalba, M ;
Monsalve, RI ;
Rodriguez, R .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1996, 97 (06) :1264-1271