Ferrocene-avidin conjugates for bioelectrochemical applications

被引：61

作者：

Padeste, C ^{[1
]}

Grubelnik, A ^{[1
]}

Tiefenauer, L ^{[1
]}

机构：

[1] Paul Scherrer Inst, Lab Micro & Nanotechnol, CH-5232 Villigen, Switzerland

来源：

BIOSENSORS & BIOELECTRONICS | 2000年 / 15卷 / 9-10期

关键词：

ferrocene; avidin; biosensors; electron transfer;

D O I：

10.1016/S0956-5663(00)00106-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Coupling of ferrocene moieties to avidin via a flexible spacer molecule yields a conjugate which combines the unique biotin-binding properties of avidin with the reversible redox characteristics of ferrocenes. Synthesis of the conjugate has been optimised and the conjugates were characterised bio- and electrochemically. Covalent immobilisation of the conjugate on gold electrodes in a dense monolayer results in electrodes with a high binding capacity for biotinylated molecules as well as good electron transfer properties. The application potential of such electrodes for bioelectrochemical systems is demonstrated by electrochemical reduction of hydrogen peroxide under mild conditions catalysed by a bound biotin-microperoxidase MP11 conjugate. (C) 2000 Elsevier Science S.A. All rights reserved.

引用

页码：431 / 438

页数：8

共 18 条

[1] COVALENT IMMOBILIZATION OF AVIDIN ON GLASSY-CARBON ELECTRODES AS THE BASIS FOR MULTIVALENT BIOSENSORS [J].

ACHTNICH, UR ;

TIEFENAUER, LX ;

ANDRES, RY .

BIOSENSORS & BIOELECTRONICS, 1992, 7 (04) :279-290

[2] AVIDIN-BIOTIN COMPLEXATION FOR ENZYME SENSOR APPLICATIONS [J].

ANZAI, J ;

HOSHI, T ;

OSA, T .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1994, 13 (05) :205-210

[3] Application of redox enzymes for probing the antigen-antibody association at monolayer interfaces: Development of amperometric immunosensor electrodes [J].

Blonder, R ;

Katz, E ;

Cohen, Y ;

Itzhak, N ;

Riklin, A ;

Willner, I .

ANALYTICAL CHEMISTRY, 1996, 68 (18) :3151-3157

[4] DIRECT ELECTRICAL COMMUNICATION BETWEEN CHEMICALLY MODIFIED ENZYMES AND METAL-ELECTRODES .1. ELECTRON-TRANSFER FROM GLUCOSE-OXIDASE TO METAL-ELECTRODES VIA ELECTRON RELAYS, BOUND COVALENTLY TO THE ENZYME [J].

DEGANI, Y ;

HELLER, A .

JOURNAL OF PHYSICAL CHEMISTRY, 1987, 91 (06) :1285-1289

[5] Direct electron transfer between heme-containing enzymes and electrodes as basis for third generation biosensors [J].

Gorton, L ;

Lindgren, A ;

Larsson, T ;

Munteanu, FD ;

Ruzgas, T ;

Gazaryan, I .

ANALYTICA CHIMICA ACTA, 1999, 400 :91-108

[6] AMPEROMETRIC GLUCOSE BIOSENSORS BASED ON REDOX POLYMER-MEDIATED ELECTRON-TRANSFER [J].

HALE, PD ;

BOGUSLAVSKY, LI ;

INAGAKI, T ;

KARAN, HI ;

LEE, HS ;

SKOTHEIM, TA ;

OKAMOTO, Y .

ANALYTICAL CHEMISTRY, 1991, 63 (07) :677-682

[7]

IKEDA T, 1992, B ELECTROCHEM, V8, P145

[8]

Kossek S, 1996, J MOL RECOGNIT, V9, P485, DOI 10.1002/(SICI)1099-1352(199634/12)9:5/6<485::AID-JMR288>3.0.CO

[9]

2-R

[10] Minizymes. A new strategy for the development of reagentless amperometric biosensors based on direct electron-transfer processes [J].

Lotzbeyer, T ;

Schuhmann, W ;

Schmidt, HL .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1997, 42 (01) :1-6

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