New primer pair for detection of Chlamydia pneumoniae by polymerase chain reaction

被引：18

作者：

Kubota, Y

机构：

[1] Division of Respiratory Diseases, Department of Medicine, Kawasaki Medical School, Kurashiki, Okayama 701-01

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1996年 / 40卷 / 01期

关键词：

Chlamydia pneumoniae; 53-kDa protein; PCR; 53.1-53.2 primer pair;

D O I：

10.1111/j.1348-0421.1996.tb03313.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In order to improve the detection and identification of Chlamydia pneumoniae, new primers for polymerase chain reaction (PCR) were designed based on the DNA base sequence within the 53-kDa protein gene, which is specific for C. pneumoniae. The primers permitted the identification of 24 C. pneumoniae strains collected from different geographical locations, but no reaction was observed with C. trachomatis, C. psittaci nor C. pecorum. The primers were unable to amplify the DNA of bacteria commonly related to respiratory tract infections. The positive amplification was achieved with only 9 EBs/assay. Therefore, the new primers seem to be useful in the diagnosis of C. pneumoniae infections.

引用

收藏

页码：27 / 32

页数：6

相关论文

共 24 条

[1] DETECTION OF CHLAMYDIA-PNEUMONIAE BY POLYMERASE CHAIN-REACTION [J].

CAMPBELL, LA ;

MELGOSA, MP ;

HAMILTON, DJ ;

KUO, CC ;

GRAYSTON, JT .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (02) :434-439

[2]

DWYER RSC, 1972, BRIT J VENER DIS, V48, P452

[3] An unidentified virus producing acute meningitis and pneumonitis in experimental animals [J].

Francis, T ;

Magill, TP .

JOURNAL OF EXPERIMENTAL MEDICINE, 1938, 68 (02) :147-U3

[4] PROPOSAL OF CHLAMYDIA-PECORUM SP-NOV FOR CHLAMYDIA STRAINS DERIVED FROM RUMINANTS [J].

FUKUSHI, H ;

HIRAI, K .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (02) :306-308

[5] CURRENT KNOWLEDGE ON CHLAMYDIA-PNEUMONIAE, STRAIN-TWAR, AN IMPORTANT CAUSE OF PNEUMONIA AND OTHER ACUTE RESPIRATORY-DISEASES [J].

GRAYSTON, JT ;

WANG, SP ;

KUO, CC ;

CAMPBELL, LA .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1989, 8 (03) :191-202

[6] CHLAMYDIA-PNEUMONIAE SP-NOV FOR CHLAMYDIA SP STRAIN TWAR [J].

GRAYSTON, JT ;

KUO, CC ;

CAMPBELL, LA ;

WANG, SP .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (01) :88-90

[7] A NEW CHLAMYDIA-PSITTACI STRAIN, TWAR, ISOLATED IN ACUTE RESPIRATORY-TRACT INFECTIONS [J].

GRAYSTON, JT ;

KUO, CC ;

WANG, SP ;

ALTMAN, J .

NEW ENGLAND JOURNAL OF MEDICINE, 1986, 315 (03) :161-168

[8] A NEW RESPIRATORY-TRACT PATHOGEN - CHLAMYDIA-PNEUMONIAE STRAIN TWAR [J].

GRAYSTON, JT ;

CAMPBELL, LA ;

KUO, CC ;

MORDHORST, CH ;

SAIKKU, P ;

THOM, DH ;

WANG, SP .

JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (04) :618-625

[9]

GRONHAGENRISKA C, 1988, SARCOIDOSIS OTHER GR, P297

[10] ASSOCIATION OF CHLAMYDIA-PNEUMONIAE (STRAIN TWAR) INFECTION WITH WHEEZING, ASTHMATIC BRONCHITIS, AND ADULT-ONSET ASTHMA [J].

HAHN, DL ;

DODGE, RW ;

GOLUBJATNIKOV, R .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1991, 266 (02) :225-230